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Abstract Although spiders are one of the most diverse groups of arthropods, the genetic architecture of their evolutionary adaptations is largely unknown. Specifically, ancient genome-wide duplication occurring during arachnid evolution ~450 mya resulted in a vast assembly of gene families, yet the extent to which selection has shaped this variation is understudied. To aid in comparative genome sequence analyses, we provide a chromosome-level genome of the Western black widow spider (Latrodectus hesperus)—a focus due to its silk properties, venom applications, and as a model for urban adaptation. We used long-read and Hi-C sequencing data, combined with transcriptomes, to assemble 14 chromosomes in a 1.46 Gb genome, with 38,393 genes annotated, and a BUSCO score of 95.3%. Our analyses identified high repetitive gene content and heterozygosity, consistent with other spider genomes, which has led to challenges in genome characterization. Our comparative evolutionary analyses of eight genomes available for species within the Araneoidea group (orb weavers and their descendants) identified 1,827 single-copy orthologs. Of these, 155 exhibit significant positive selection primarily associated with developmental genes, and with traits linked to sensory perception. These results support the hypothesis that several traits unique to spiders emerged from the adaptive evolution of ohnologs—or retained ancestrally duplicated genes—from ancient genome-wide duplication. These comparative spider genome analyses can serve as a model to understand how positive selection continually shapes ancestral duplications in generating novel traits today within and between diverse taxonomic groups. 

Frequently, users on the web need to show that they are, for example, not a robot, old enough to access an age restricted video, or eligible to download an ebook from their local public library without being tracked. Anonymous credentials were developed to address these concerns. However, existing schemes do not handle the realities of deployment or the complexities of real-world identity. Instead, they implicitly make assumptions such as there being an issuing authority for anonymous credentials that, for real applications, requires the local department of motor vehicles to issue sophisticated cryptographic tokens to show users are over 18. In reality, there are multiple trust sources for a given identity attribute, their credentials have distinctively different formats, and many, if not all, issuers are unwilling to adopt new protocols.We present and build zk-creds, a protocol that uses general-purpose zero-knowledge proofs to 1) remove the need for credential issuers to hold signing keys: credentials can be issued to a bulletin board instantiated as a transparency log, Byzantine system, or even a blockchain; 2) convert existing identity documents into anonymous credentials without modifying documents or coordinating with their issuing authority; 3) allow for flexible, composable, and complex identity statements over multiple credentials. Concretely, identity assertions using zk-creds take less than 150ms in a real-world scenario of using a passport to anonymously access age-restricted videos.